(1) Run a leak check from the maintenance screen and follow the onscreen instructions. If it fails the leak check, first ensure that the cap is correctly aligned on the top of the sample tube. If it is not, follow
this procedure before continuing. Assuming the cap is correctly aligned, exit the maintenance screen and proceed to put 5 mL of DI water into a sample tube and place in position 11 and simulate a sample run with the software for position 11. You will now be watching for bubbles, or lack thereof, throughout the entire run.
(a) If bubbling occurs during the "Purge" step before measurement.
--> There is a leak between the V1 valve and the Yellow-marked tube, check the connections at both ends.
Or the V3 valve is not working. The V3 valve can be checked in the maintenance screen, listen for actuation as
you toggle it on and off.
(b) If bubbling does not occur in the waste station during the "Rinse" step
--> There is a leak between the discharge side of the air pump and the yellow-marked tube.
(c) If bubbling does not occur in the sample tube during measurement check for the following:
--> (i) A crack or chip in the sample tube
(ii) foreign material on the sealing gasket of the cap
(iii) Leak where the tubing enters the cap, make sure all the tubing is fully inserted into the cap and do not
slide easily
(iv) Leak at the dehumidifier or dehumidifier drain tank
(v) Leak in the flowcell
(vi) Faulty V3 Valve